IPTG诱导表达的作用原理是什么?

Question

如题所述

Answer 1

IPTG（异丙基硫代-β-D-半乳糖）是乳糖操纵子的诱导物，将其加入到培养基后，能够与lacI产生的阻遏蛋白结合，使转录酶能够顺利通过操纵基因，外源基因大量转录并高效表达。

Answer 2

Binding of the repressor-IPTG complex to the operator can be studied by using greater concentrations of the protein in the methylation protection/enhancement assay. The large amount compensates for the low affinity of the repressor-IPTG complex for the operator. The complex makes exactly the same pattern of contacts with DNA as the free repressor. An analogous result is obtained with mutant repressors whose affinity for operator DNA is increased; they too make the same pattern of contacts.

参考资料：gene 7

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